Primary Identifier | MGI:2387865 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Otp |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Sequence encoding the third helix of the homeodomain was disrupted by the insertion of a lacZ reporter cassette. The inserted cassette contained stop codons in all three reading frames, precluding the translation of the endogenous carboxy terminal, which is involved in transactivation. Comparisons with wild-type in situ hybridization results showed that the expression of lacZ was similar to the endogenous expression pattern. |