| First Author | Morishige K | Year | 1994 |
| Journal | FEBS Lett | Volume | 346 |
| Issue | 2-3 | Pages | 251-6 |
| PubMed ID | 8013643 | Mgi Jnum | J:18699 |
| Mgi Id | MGI:66938 | Doi | 10.1016/0014-5793(94)00483-8 |
| Citation | Morishige K, et al. (1994) Molecular cloning and functional expression of a novel brain-specific inward rectifier potassium channel. FEBS Lett 346(2-3):251-6 |
| abstractText | We have cloned a novel brain-specific inward rectifier K+ channel from a mouse brain cDNA library and designated it MB-IRK3. The mouse brain cDNA library was screened using a fragment of the mouse macrophage inward rectifier K+ channel (IRK1) cDNA as a probe. The amino acid sequence of MB-IRK3 shares 61% and 64% identity to MB-IRK1 and RB-IRK2, respectively. Xenopus oocytes injected with cRNA derived from this clone expressed a potassium current which showed inward-rectifying channel characteristics similar to MB-IRK1 and RB-IRK2 currents, but distinct from ROMK1 or GIRK1 current. However, the single channel conductance of MB-IRK3 was approximately 10 pS with 140 mM extracellular K+, which was distinct from that of MB-IRK1 (20 pS). MB-IRK3 mRNA expressed specifically in the forebrain, which clearly differed from MB-IRK1 and RB-IRK2 mRNAs. These results indicate that members of the IRK family with distinct electrophysiological properties express differentially and may play heterogeneous functional roles in brain functions. |
