| First Author | Shinohara K | Year | 2012 |
| Journal | Nat Commun | Volume | 3 |
| Pages | 622 | PubMed ID | 22233632 |
| Mgi Jnum | J:220930 | Mgi Id | MGI:5637476 |
| Doi | 10.1038/ncomms1624 | Citation | Shinohara K, et al. (2012) Two rotating cilia in the node cavity are sufficient to break left-right symmetry in the mouse embryo. Nat Commun 3:622 |
| abstractText | Determination of left-right asymmetry in mouse embryos is achieved by a leftward fluid flow (nodal flow) in the node cavity that is generated by clockwise rotational movement of 200-300 cilia in the node. The precise action of nodal flow and how much flow input is required for the robust read-out of left-right determination remains unknown. Here we show that a local leftward flow generated by as few as two rotating cilia is sufficient to break left-right symmetry. Quantitative analysis of fluid flow and ciliary rotation in the node of mouse embryos shows that left-right asymmetry is already established within a few hours after the onset of rotation by a subset of nodal cilia. Examination of various ciliary mutant mice shows that two rotating cilia are sufficient to initiate left-right asymmetric gene expression. Our results suggest the existence of a highly sensitive system in the node that is able to sense an extremely weak unidirectional flow, and may favour a model in which the flow is sensed as a mechanical force. |
