| First Author | Murakami E | Year | 2016 |
| Journal | PLoS One | Volume | 11 |
| Issue | 9 | Pages | e0162997 |
| PubMed ID | 27643686 | Mgi Jnum | J:289450 |
| Mgi Id | MGI:6253974 | Doi | 10.1371/journal.pone.0162997 |
| Citation | Murakami E, et al. (2016) Cloning and Characterization of Novel Testis-Specific Diacylglycerol Kinase eta Splice Variants 3 and 4. PLoS One 11(9):e0162997 |
| abstractText | Diacylglycerol kinase (DGK) phosphorylates DG to generate phosphatidic acid. Recently, we found that a new alternative splicing product of the DGKeta gene, DGKeta3, which lacks exon 26 encoding 31 amino acid residues, was expressed only in the secondary spermatocytes and round spermatids of the testis. In this study, we cloned the full length DGKeta3 gene and confirmed the endogenous expression of its protein product. During the cloning procedure, we found a new testis-specific alternative splicing product of the DGKeta gene, DGKeta4, which lacks half of the catalytic domain. We examined the DGK activity and subcellular localization of DGKeta3 and eta4. DGKeta3 had almost the same activity as DGKeta1, whereas the activity of DGKeta4 was not detectable. In resting NEC8 cells (human testicular germ cell tumor cell line), DGKeta1, eta3 and eta4 were broadly distributed in the cytoplasm. When osmotically shocked, DGKeta1 and eta4 were distributed in punctate vesicles in the cytoplasm. In contrast, DGKeta3 was partly translocated to the plasma membrane and co-localized with the actin cytoskeleton. These results suggest that DGKeta3 and eta4 have properties different from those of DGKeta1 and that they play roles in the testis in a different manner. |
