Primary Identifier | MGI:1933381 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Pou5f1 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | An internal ribosome entry site-lacZ-neomycin resistance cassette (IRES-beta-geo) replaced sequences in exons 2-5 encoding the two DNA binding domains and the carboxy terminal transactivation site. Immunostaining did not detect wild-type protein in 3 of 15 2.5 day morulae from an intercross of heterozygous mutant animals. |