First Author | Palfree RG | Year | 1988 |
Journal | J Immunol | Volume | 140 |
Issue | 1 | Pages | 305-10 |
PubMed ID | 3335781 | Mgi Jnum | J:8979 |
Mgi Id | MGI:57443 | Doi | 10.4049/jimmunol.140.1.305 |
Citation | Palfree RG, et al. (1988) N-terminal and cDNA characterization of murine lymphocyte antigen Ly-6C.2. J Immunol 140(1):305-10 |
abstractText | The Ly-6C.2 molecule was purified from K36 tumor cells by affinity chromatography and gel filtration. The electrophoretically homogeneous preparation, with m.w. 15,000, was tested with a panel of antibodies that confirmed the presence of the LY-6C.2 epitope. An N-terminal sequence of 39 amino acids was obtained showing 59% homology with the corresponding portion of the Ly-6A.2 polypeptide. Based on the least homologous (29%) 14 amino acid segment, an oligonucleotide probe was constructed, and Ly-6C.2 cDNA was cloned from a BW5147 cDNA library. A 794-base pair cDNA containing the entire coding region had 82% homology with Ly-6A.2 cDNA. The encoded polypeptide sequence of 131 amino acids containing a perfect correlation with the N-terminal sequence data was 63% homologous with that of Ly-6A.2. The greatest homology was in the leader, first 16 N-terminal and last 39 C-terminal amino acids. The latter are likely to be important in determining the attachment of glycophosphatidylinositol. Despite results indicating fewer disulfide constraints in the Ly-6C molecule, the predicted sequence contains 10 cysteine residues nearly perfectly matched with those predicted in Ly-6A. |