| First Author | de Lange WJ | Year | 2008 |
| Journal | Physiol Genomics | Volume | 35 |
| Issue | 1 | Pages | 1-4 |
| PubMed ID | 18612081 | Mgi Jnum | J:138838 |
| Mgi Id | MGI:3806569 | Doi | 10.1152/physiolgenomics.90284.2008 |
| Citation | de Lange WJ, et al. (2008) Germ line activation of the Tie2 and SMMHC promoters causes noncell-specific deletion of floxed alleles. Physiol Genomics 35(1):1-4 |
| abstractText | Tissue-specific knockouts generated through Cre-loxP recombination have become an important tool to manipulate the mouse genome. Normally, two successive rounds of breeding are performed to generate mice carrying two floxed target-gene alleles and a transgene expressing Cre-recombinase tissue-specifically. We show herein that two promoters commonly used to generate endothelium-specific (Tie2) and smooth muscle-specific [smooth muscle myosin heavy chain (Smmhc)] knockout mice exhibit activity in the female and male germ lines, respectively. This can result in the inheritance of a null allele in the second generation that is not tissue specific. Careful experimental design is required therefore to ensure that tissue-specific knockouts are indeed tissue specific and that appropriate controls are used to compare strains. |
