Primary Identifier | MGI:5707374 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Ltf |
Transmission | Germline | Strain of Origin | 129 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted upstream of exon 3. An additional loxP site was inserted downstream of exon 3. Flp-mediated recombination removed the selection cassette and left exon 3 floxed. Cre-mediated recombination removed exon 3. Western blot analysis confirmed the absence of protein expression in bone marrow. |