| First Author | Friedrich G | Year | 1991 |
| Journal | Genes Dev | Volume | 5 |
| Issue | 9 | Pages | 1513-23 |
| PubMed ID | 1653172 | Mgi Jnum | J:79478 |
| Mgi Id | MGI:2388332 | Doi | 10.1101/gad.5.9.1513 |
| Citation | Friedrich G, et al. (1991) Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice. Genes Dev 5(9):1513-23 |
| abstractText | A general strategy for selecting insertion mutations in mice has been devised. Constructs lacking a promoter and including a beta-galactosidase gene, or a reporter gene encoding a protein with both beta-galactosidase and neomycin phosphotransferase activity, were designed so that activation of the reporter gene depends on its insertion within an active transcription unit. Such insertion events create a mutation in the tagged gene and allow its expression to be followed by beta-galactosidase activity. Introduction of promoter trap constructs into embryonic stem (ES) cells by electroporation or retroviral infection has led to the derivation of transgenic lines that show a variety of beta-galactosidase expression patterns. Intercrossing of heterozygotes from 24 strains that express beta-galactosidase identified 9 strains in which homozygosity leads to an embryonic lethality. Because no overt phenotype was detected in the remaining strains, these results suggest that a substantial proportion of mammalian genes identified by this approach are not essential for development. |
