| First Author | He J | Year | 2017 |
| Journal | Arch Biochem Biophys | Volume | 625-626 |
| Pages | 30-38 | PubMed ID | 28579197 |
| Mgi Jnum | J:249295 | Mgi Id | MGI:6094038 |
| Doi | 10.1016/j.abb.2017.05.020 | Citation | He J, et al. (2017) miR-491 inhibits skeletal muscle differentiation through targeting myomaker. Arch Biochem Biophys 625-626:30-38 |
| abstractText | The myogenesis of skeletal muscle has several stages, including satellite cell proliferation, differentiation, fusion and specific muscle formation. Recent studies have shown that myomaker, a muscle-specific transmembrane protein, was critical for myoblasts fusion. However, the regulatory mechanism of myomaker and its effects on myogenesis remain elusive. In this study, miR-491 was identified as a post-transcriptional regulator of myomaker, which binds specifically to its 3' untranslated region leading to its down-regulation. At the end of myotube differentiation, the expression levels of miR-491 increased drastically, while myomaker was significantly down-regulated, which indicated that miR-491 shut down the expression of myomaker. Functional studies showed that miR-491 overexpression suppressed muscle cell differentiation and adult muscle regeneration, while the inhibition of miR-491 promoted myotube differentiation. Taken together, our findings identified miR-491 as a novel negative regulator of myogenic differentiation through targeting myomaker. |
