| First Author | Kim JY | Year | 2007 |
| Journal | Arch Biochem Biophys | Volume | 464 |
| Issue | 1 | Pages | 100-11 |
| PubMed ID | 17559793 | Mgi Jnum | J:126879 |
| Mgi Id | MGI:3762266 | Doi | 10.1016/j.abb.2007.04.018 |
| Citation | Kim JY, et al. (2007) Differentiation-dependent expression of Adhfe1 in adipogenesis. Arch Biochem Biophys 464(1):100-11 |
| abstractText | We have determined that adipocytes are a major site of expression of the transcript for the novel alcohol dehydrogenase (ADH), Adhfe1. Adhfe1 is unique in that the sequence of its encoded protein places it among the iron-activated ADHs. Western blot analysis reveals Adhfe1 encodes a 50 kDa protein and immunocytochemical staining indicates mitochondrial localization. Adhfe1 transcript exhibits differentiation-dependent expression during in vitro brown and white adipogenesis. Unlike many adipocyte-enriched genes, however, Adhfe1 transcript expression in adipocytes is refractory to TNFalpha-mediated downregulation. However, use of pharmacological inhibitors reveals PI 3-kinase-mediated signals maintain the basal level of Adhfe1 transcript in 3T3-L1 adipocytes. Tissue profiling studies show Adhfe1 transcript is restricted to white and brown adipose tissues, liver, and kidney. In comparison to C57BL/6 mice, Adhfe1 transcript is downregulated 40% in white adipose tissue of ob/ob obese mice. Further characterization of Adhfe1 should yield new insights into adipocyte function and energy metabolism. |
