| First Author | Johnson KD | Year | 2007 |
| Journal | Blood | Volume | 109 |
| Issue | 12 | Pages | 5230-3 |
| PubMed ID | 17339418 | Mgi Jnum | J:145421 |
| Mgi Id | MGI:3834540 | Doi | 10.1182/blood-2007-02-072983 |
| Citation | Johnson KD, et al. (2007) Friend of GATA-1-independent transcriptional repression: a novel mode of GATA-1 function. Blood 109(12):5230-3 |
| abstractText | The GATA-1-interacting protein Friend Of GATA-1 (FOG-1) is essential for the proper transcriptional activation and repression of numerous GATA-1 target genes. Although FOG-1-independent activation by GATA-1 has been described, all known examples of GATA-1-mediated repression are FOG-1 dependent. In the GATA-1-null G1E cell line, estrogen receptor ligand binding domain (ER) chimeras of either wild-type GATA-1 or a FOG-1-binding defective mutant of GATA-1 repressed several genes similarly upon activation with beta-estradiol. Repression also occurred in a FOG-1-null cell line expressing ER-GATA-1 and during ex vivo erythropoiesis. At the Lyl1 and Rgs18 loci, we found highly restricted occupancy by GATA-1 and GATA-2, indicating that these genes are direct targets of GATA factor regulation. The identification of genes repressed by GATA-1 independent of FOG-1 defines a novel mode of GATA-1-mediated transcriptional regulation. |
