| First Author | Hamada Y | Year | 2010 |
| Journal | Biochem Biophys Res Commun | Volume | 392 |
| Issue | 2 | Pages | 166-70 |
| PubMed ID | 20059976 | Mgi Jnum | J:157869 |
| Mgi Id | MGI:4437184 | Doi | 10.1016/j.bbrc.2010.01.001 |
| Citation | Hamada Y, et al. (2010) The pro-alpha2(XI) collagen gene is expressed in odontoblasts. Biochem Biophys Res Commun 392(2):166-70 |
| abstractText | Since the dentine is analogous to bone, its extracellular matrix shares many similarities to bone tissues. Similar to the bone, type I collagen is the major organic component in dentine. However, little is known about minor fibrillar collagens, which seem to be co-expressed such as type I or II collagen. The present study examined the gene expression of type V and XI collagens, which play important roles in collagen fibril formation and skeletal morphogenesis, using RT-PCR and in situ hybridization combined with immunohistochemistry. The transcripts of pro-alpha1(XI), pro-alpha2(XI), pro-alpha1(V) and pro-alpha2(V) chains were present, but not pro-alpha3(V) and pro-alpha1(II) chains, of which an overglycosylated variant is pro-alpha3(XI) chain, in mouse incisor tooth, using RT-PCR and in situ hybridization. The pro-alpha2(XI) protein, which is mainly expressed in cartilage, were observed in the odontoblast using a specific polyclonal antibody. Real-time RT-PCR showed that the transcripts of pro-alpha2(XI), pro-alpha1(V) and pro-alpha2(V) were predominant in crown and that of pro-alpha1(XI) in root of the tooth. Finally, the expression of pro-alpha2(XI) was confirmed with an odontoblastic cell line transformed with human telomerase reverse transcriptase (hTERT) both in vitro and in vivo. The data suggest a new subtype of the V/XI collagen molecule containing alpha2(XI) chain. |
