Primary Identifier | MGI:2177822 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Eng |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | An HA tag followed by a stop codon was introduced into exon 8, and was followed by an IRES-lacZ gene and neomycin selection cassette that replaced exons 9, 10 and 11. Western blot analysis on homozygous embryos demonstrated that no detectable ENG protein was expressed from this allele; however, a beta-galactosidase protein was expressed under the control of the endogenous promoter. |