| Primary Identifier | IPR020604 | Type | Domain |
| Short Name | CTF/NFI_DNA-bd-dom |
| description | Nuclear factor I (NF-I) or CCAAT box-binding transcription factor (CTF) [, , ](also known as TGGCA-binding proteins) are a family of vertebrate nuclear proteins which recognise and bind, as dimers, the palindromic DNA sequence 5'-TGGCANNNTGCCA-3'. This family was first described for its role in stimulating the initiation of adenovirus DNA replication []. In vertebrates there are four members NFIA, NFIB, NFIC, and NFIX and an orthologue from Caenorhabditis elegans has been described, called Nuclear factor I family protein (NFI-I) []. The CTF/NF-I proteins are individually capable of activating transcription and DNA replication, thus they function by regulating cell proliferation and differentiation. They are involved in normal development and have been associated with developmental abnormalities and cancer in humans []. In a given species, there are a large number of different CTF/NF-I proteins, generated both by alternative splicing and by the occurrence of four different genes. CTF/NF-1 proteins contain 400 to 600 amino acids. The N-terminal 200 amino-acid sequence, almost perfectly conserved in all species and genes sequenced, mediates site-specific DNA recognition, protein dimerisation and Adenovirus DNA replication. The C-terminal 100 amino acids contain the transcriptional activation domain. This activation domain is the target of gene expression regulatory pathways elicited by growth factors and it interacts with basal transcription factors and with histone H3 [].This entry represents the 200 amino-acid DNA-binding domain found in N-terminal of CTF/NF1 proteins. It mediates site-specific DNA recognition, protein dimerisation and Adenovirus DNA replication. The CTF/NF-I DNA-binding domain contains four conserved Cys residues, which are required for its DNA-binding activity []. |
