Primary Identifier | MGI:2653126 | Allele Type | Targeted |
Attribute String | Not Specified | Gene | Krt14 |
Transmission | Germline | Strain of Origin | 129X1/SvJ |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A C to T point mutation at codon 131 was introduced to the gene and a floxed PGK-neo cassette was inserted into the downstream exon via homologous recombination. The point mutation results in an arginine to cysteine substitution. The targeted allele was confirmed by Southern blot and sequence analysis. |