Primary Identifier | MGI:5296243 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Gata5 |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | An FRT-flanked neo cassette with a 5' loxP site was inserted upstream of exon 3. An additional loxP site was inserted downstream of exon 6. Cre-mediated recombination removed exons 3 through 6 and the neo cassette. The absence of transcripts containing exons 4 through 6 and 90% reduction in transcripts with exons 1 and 2 were confirmed by quantitative RT-PCR analysis on E12.5 heart extracts. |