| First Author | Andres AC | Year | 1994 |
| Journal | Oncogene | Volume | 9 |
| Issue | 5 | Pages | 1461-7 |
| PubMed ID | 8152808 | Mgi Jnum | J:17851 |
| Mgi Id | MGI:65877 | Citation | Andres AC, et al. (1994) Expression of two novel eph-related receptor protein tyrosine kinases in mammary gland development and carcinogenesis [published erratum appears in Oncogene 1994 Aug;9(8):2431]. Oncogene 9(5):1461-7 |
| abstractText | To investigate the involvement of protein tyrosine kinases (PTKs) in the growth control of mammary epithelial cells, we have used PCR based cloning to identify PTKs expressed in a mouse mammary epithelial cell line. This approach led to the isolation of two receptor PTKs of the eph-related subfamily; myk-1, a novel member expressed predominantly in lung, heart and mammary gland and myk-2, a close relative of the human eck gene. Northern blot analysis of RNA from mouse mammary glands at different stages of development revealed that myk-1 and myk-2 expression is induced at puberty and differentially regulated during the estrus cycle. myk-1 and myk-2 expression was down-regulated during the pregnancy induced differentiation of the mammary gland. Over-expression of myk-1 and myk-2 was found in the undifferentiated and invasive mammary tumors of transgenic mice expressing the Ha-ras oncogene. In contrast, no elevated expression of either gene could be detected in the well differentiated and non-metastatic mammary tumors of c-myc expressing transgenic mice. These results indicate that myk-1 and myk-2 expression is induced during the proliferation of the mammary gland and down-regulated by its differentiation. |
