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Allele : Tg(GFAP-CHRM3*)6Kdmc transgene insertion 6, Kevin D McCarthy
Primary Identifier  MGI:6148045 Allele Type  Transgenic
Attribute String  Inserted expressed sequence Gene  Tg(GFAP-CHRM3*)6Kdmc
Strain of Origin  C3H/HeJ x C57BL/6J Is Recombinase  false
Is Wild Type  false
molecularNote  This transgene expresses a modified human CHRM3 sequence (DREADD or hM3dq) under the control of a human GFAP promoter.
The hM3Dq ("DREADD") sequence is a Gq-coupled human M3 muscarinic DREADD (designer receptor exclusively activated by designer drug). To create the hM3Dq sequence, the wildtype human muscarinic 3 receptor (CHRM3) sequence was modified via site-directed mutagenesis to harbor two amino acid substitutions (Y149C<3.33>/A239G<5.46>) that abolish receptor affinity for the native ligand, acetylcholine (ACh), but allow receptor binding and subsequent activation by the small drug-like molecule clozapine-N-oxide (CNO).
  • mutations:
  • Insertion
  • synonyms:
  • GFAP-hM3Dq,
  • GFAP-Gq-DREADD,
  • GFAP-DREADD 3,
  • GFAP-hM3Dq,
  • GFAP-DREADD 3,
  • GFAP-Gq-DREADD
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1 Feature

Trail: Allele

Genome

1 Expresses

Trail: Allele

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

1 Carried By

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0 Driven By

9 Publication categories

Trail: Allele




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