| First Author | Kay PH | Year | 1987 |
| Journal | J Immunogenet | Volume | 14 |
| Issue | 4-5 | Pages | 247-53 |
| PubMed ID | 2900855 | Mgi Jnum | J:9321 |
| Mgi Id | MGI:57783 | Doi | 10.1111/j.1744-313x.1987.tb00387.x |
| Citation | Kay PH, et al. (1987) Heterogeneity and linkage of equine C4 and steroid 21-hydroxylase genes. J Immunogenet 14(4-5):247-53 |
| abstractText | The fourth component of complement (C4) is polymorphic in most species studied, and is encoded by a gene or genes within the MHC. In man and mouse there are two closely linked C4 and steroid 21-hydroxylase (21-OH) genes. Therefore we have used Southern blotting to determine whether equine C4 and 21-OH genes are linked. C4 restriction fragment length polymorphism (RFLP) was found with the enzymes EcoRI and BamHI. Comparison of the sizes of EcoRI-digested fragments of genomic DNA hybridizing with C4 and 21-OH probes revealed that equine C4 and 21-OH genes are separated by no more than 13 kb. Further, there is no evidence of C4 and 21-OH gene duplication in the horse. Segregation of ELA and different polymorphic forms of equine C4 suggest that C4 and 21-OH genes are within the MHC. It is likely that equine MHC supratypes will provide improved markers of disease susceptibility. |
