| First Author | Siewe BT | Year | 2011 |
| Journal | Blood | Volume | 117 |
| Issue | 14 | Pages | 3770-9 |
| PubMed ID | 21285437 | Mgi Jnum | J:172852 |
| Mgi Id | MGI:5009118 | Doi | 10.1182/blood-2010-08-301119 |
| Citation | Siewe BT, et al. (2011) In vitro requirement for periostin in B lymphopoiesis. Blood 117(14):3770-9 |
| abstractText | B lymphopoiesis arrests in rabbits by 4 months of age. To identify molecules that contribute to this arrest, cDNA-representational difference analysis on BM stromal cells from young and adult rabbits showed that expression of Postn that encodes for the extracellular matrix protein periostin dramatically reduced with age. Postn-small interfering RNA OP9 cells lost their capacity to support B-cell development from rabbit or murine BM cells, and reexpression of periostin restored this potential, indicating an in vitro requirement for periostin in B lymphopoiesis. In our system, we determined that periostin deficiency leads to increased cell death and decreased proliferation of B-lineage progenitors. Further, RGD peptide inhibition of periostin/alpha(v)beta(3) interaction resulted in a marked decrease in B lymphopoiesis in vitro. Microarray analysis of the Postn-small interfering RNA OP9 cells showed decreased expression of key B-lymphopoietic factors, including IL-7 and CXCL12. In vivo, unidentified molecule(s) probably compensate periostin loss because Postn(-/-) mice had normal numbers of B-cell progenitors in BM. We conclude that the decline in periostin expression in adult rabbit BM does not solely explain the arrest of B lymphopoiesis. However, the interaction of periostin with alpha(v)beta(3) on lymphoid progenitors probably provides both proliferative and survival signals for cells in the B-cell development pathway. |
