| First Author | Shirakami Y | Year | 2023 |
| Journal | Int J Mol Sci | Volume | 24 |
| Issue | 19 | PubMed ID | 37834032 |
| Mgi Jnum | J:341622 | Mgi Id | MGI:7542170 |
| Doi | 10.3390/ijms241914581 | Citation | Shirakami Y, et al. (2023) A Novel Mouse Model of Intrahepatic Cholangiocarcinoma Induced by Azoxymethane. Int J Mol Sci 24(19) |
| abstractText | Cholangiocarcinoma is the second most common primary cancer of the liver and has a poor prognosis. Various animal models, including carcinogen-induced and genetically engineered rodent models, have been established to clarify the mechanisms underlying cholangiocarcinoma development. In the present study, we developed a novel mouse model of malignant lesions in the biliary ducts induced by the administration of the carcinogen azoxymethane to obese C57BLKS/J-db/db mice. A histopathological analysis revealed that the biliary tract lesions in the liver appeared to be an intrahepatic cholangiocarcinoma with higher tumor incidence, shorter experimental duration, and a markedly increased incidence in obese mice. Molecular markers analyzed using a microarray and a qPCR indicated that the cancerous lesions originated from the cholangiocytes and developed in the inflamed livers. These findings indicated that this is a novel mouse model of intrahepatic cholangiocarcinoma in the context of steatohepatitis. This model can be used to provide a better understanding of the pathogenic mechanisms of cholangiocarcinoma and to develop novel therapeutic strategies for this malignancy. |
