| First Author | Poole SJ | Year | 1989 |
| Journal | Genomics | Volume | 4 |
| Issue | 3 | Pages | 225-31 |
| PubMed ID | 2565873 | Mgi Jnum | J:9752 |
| Mgi Id | MGI:58209 | Doi | 10.1016/0888-7543(89)90324-8 |
| Citation | Poole SJ, et al. (1989) Isolation and chromosomal localization of the human En-2 gene. Genomics 4(3):225-31 |
| abstractText | By low stringency hybridization we have isolated from a human cosmid genomic library sequences homologous with a probe from the Drosophila engrailed gene. Partial nucleotide sequence analysis shows a consensus splice acceptor site followed by an open reading frame (ORF) that can encode 104 amino acids; the first 94 amino acids have 71% identity with the Drosophila engrailed protein. The shared region contains a homeo domain and is within the region of engrailed shared with the Drosophila invected gene and the mouse En-1 and En-2 genes. At the amino acid level, the human sequence is 85% identical with the mouse En-1 gene and 100% identical with the mouse En-2 gene. Hybridization against a panel of human-hamster somatic cell hybrids maps this human En-2 gene to chromosome 7, and regional mapping by in situ hybridization to human chromosomes localizes it to region 7q36 at the end of the long arm. |
