Primary Identifier | MGI:5708309 | Allele Type | Targeted |
Gene | Lamb3 | Transmission | Germline |
Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | A 628G>A mutation was inserted in exon 7 and an FRT flanked neo cassette was introduced in intron 8 via homologous recombination. Flp mediated recombination removed the neo cassette. PCR analysis indicates the production of a shorter transcript with deletion of exon 7 resulting in a frameshift and premaure stop codon (p.V189NfsX8). |