| Primary Identifier | MGI:6307033 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Tmem33 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences ACTTGCTCACACATATGCTA and GACTGCTCGTTGAAAAGCTG, which resulted in a 453 bp deletion beginning at Chromosome 5 position 67,263,561 bp and ending after 67,264,013 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001232921 (exon 3) and 358 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 15 and early truncation 2 amino acids later. There is a single bp (A) insert at the deletion site. |
