Primary Identifier | MGI:5645747 | Allele Type | Transgenic |
Attribute String | Inserted expressed sequence | Gene | Tg(ADCYAP1R1)1Sshe |
Strain of Origin | C57BL/6 or CBA/Ca | Is Recombinase | false |
Is Wild Type | false |
description | "PAC1-overexpressing mice were generated in a mixed genetic background of CBA/Ca: C57Bl/6...." (J:179496) |
molecularNote | The transgenic construct comprised the 130-kb RPCI PAC clone 204D22, containing the human adenylate cyclase activating polypeptide 1 receptor 1 (ADCYAP1R1; also called PAC1) gene, modified by insertion of an internal ribosomal entry site (IRES) followed by the lacZ coding sequence (IRES-lacZ-p[A] cassette) into the 3' untranslated region (3' UTR) downstream of the human gene's polyadenylation signal. Semi-quantitative PCR of genomic DNA from heterozygous mice determined that transgenic line 1 (also called A149) carries six copies of the transgene. RT-PCR of RNA from embryonic day 10.5 (E10.5) embryos revealed a high level of transgene expression. |