| First Author | Park KC | Year | 2014 |
| Journal | Biochem Biophys Res Commun | Volume | 443 |
| Issue | 4 | Pages | 1291-5 |
| PubMed ID | 24406168 | Mgi Jnum | J:218144 |
| Mgi Id | MGI:5616716 | Doi | 10.1016/j.bbrc.2013.12.140 |
| Citation | Park KC, et al. (2014) Regulation of mIkappaBNS stability through PEST-mediated degradation by proteasome. Biochem Biophys Res Commun 443(4):1291-5 |
| abstractText | Negative regulatory proteins in a cytokine signaling play a critical role in restricting unwanted excess activation of the signaling pathway. At the same time, negative regulatory proteins need to be removed rapidly from cells to respond properly to the next incoming signal. A nuclear IkappaB protein called IkappaBNS is known to inhibit a subset of NF-kappaB target genes upon its expression by NF-kappaB activation. Here, we show a mechanism to control the stability of mIkappaBNS which might be important for cells to prepare the next round signaling. We found that mIkappaBNS is a short-lived protein of which the stability is controlled by proteasome, independent of ubiquitylation process. We identified that the N-terminal PEST sequence in mIkappaBNS was critical for the regulation of stability. |
