| Primary Identifier | MGI:3849324 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Trpc5 |
| Transmission | Germline | Strain of Origin | 129S1/SvImJ |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A single loxP site before exon 5 and a PGK-neo cassette flanked by two frt sites followed by a loxP site after exon 5 was introduced into the locus via homologous recombination. The exon 5 genomic region encoding the putative 4th transmembrane domain was deleted in ES cells by transient cre expression. RT-PCR analysis of transcripts from whole brain confirmed the absence of exon 5. Immunoprecipitation and Western blot analyses confirmed loss of protein product. |
