| Primary Identifier | MGI:2180803 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Kif1c |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A cassette consisting of a splice acceptor site, an internal ribosomal entry site (IRES), and a lacZ-neomycin resistance gene fusion replaced 2.6 kb of sequence encoding the ATP binding motif P-loop. The cassette was inserted downstream of the ATG-containing exon. A beta-galactosidase fusion protein is expressed from this allele. Immunoblot analysis using a polyclonal antibody against a C-terminal peptide sequence did not detect wild-type protein in brain from homozygous mutant mice. |
