| Primary Identifier | MGI:2680817 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence | Gene | Msh2 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The incorporation of an engineered point mutation in exon 13 created a glycine to alanine substitution at codon 674 (G674A), within the conserved ATPase domain of the C-terminal. A floxed neo-PGK-hygro cassette that was included in the targeting vector was removed by cre-mediated recombination leaving a single loxP site and the G674A mutation. Western blot analysis of homozygous mutant MEFs indicated expression of the mutant protein at wild-type levels. |
