Primary Identifier | MGI:4398911 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Steap3 |
Transmission | Germline | Strain of Origin | C57BL/6 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A 46 bp polynucleotide containing a LoxP site was added 404bp upstream exon 2, and a neomycin resistance gene floxed by two LoxP sites was added 533bp downstream exon 3. Cre-mediated excision of exons 2-3 was achieved upon electroporation of a Cre-recombinase expression vector in the correctly targeted ES clones. Protein was undetected in homozygous mutant mice by Western blot analysis and Immnunohistological staining. |