| First Author | Chun T | Year | 1999 |
| Journal | J Immunol | Volume | 162 |
| Issue | 11 | Pages | 6562-71 |
| PubMed ID | 10352272 | Mgi Jnum | J:55070 |
| Mgi Id | MGI:1337201 | Doi | 10.4049/jimmunol.162.11.6562 |
| Citation | Chun T, et al. (1999) Molecular cloning and characterization of a novel CD1 gene from the pig. J Immunol 162(11):6562-71 |
| abstractText | Much effort is underway to define the immunological functions of the CD1 multigene family; which encodes a separate lineage of Ag presentation molecules capable of presenting lipid and glycolipid Ags, To identify porcine CD1 homologues, a cosmid library was constructed and screened with a degenerate CD1 alpha 3 domain probe. One porcine CD1 gene (pCD1.1) was isolated and fully characterized, The pCD1.1 gene is organized similarly to MHC class I and other CD1 genes and contains an open reading frame of 1020 bp encoding 339 amino acids. Expression of pCD1.1 mRNA was observed in CD3(-) thymocytes, B lymphocytes, and tissue macrophages and dendritic cells. The pCD1.1 cDNA was transfected into Chinese hamster ovary cells, and subsequent FAGS analysis demonstrated that mAb 76-7-4, previously suggested to be a pig CD1 mAb, recognizes cell surface pCD1.1. Structurally, the pCD1.1 alpha 1 and alpha 2 domains are relatively dissimilar to those of other: CD1 molecules, whereas the alpha 3 domain is conserved. Overall, pCD1.1 bears the highest similarity with human CD1a, and the ectodomain sequences characteristically encode a hydrophobic Ag- binding pocket. Distinct from other CD1 molecules, pCD1.1 contains a putative serine phosphorylation moth similar to that found in human, pig, and mouse MHC class Ia molecules and to that found in rodent, but not human, MHC class-I related (MR1) cytoplasmic tail sequences. Thus, pCD1.1 encodes a molecule with a conventional. CD1 ectodomain and an MHC class I-like cytoplasmic tail. The unique features of pCD1.1 provoke intriguing questions about the immunologic functions of CD1 and the evolution of Ag presentation gene families. |
