| Primary Identifier | MGI:4834777 | Allele Type | Targeted |
| Gene | Hprt1 | Transmission | Germline |
| Strain of Origin | (B6.129P2-Hprt1<b-m3>/J x 129S-Gt(ROSA)26Sor<tm1Sor>/J)F1 | Is Recombinase | false |
| Is Wild Type | false | Project Collection | Pleiades Promoter Project |
| description | Germ line transmission of mutant cell line mEMS3689 has been established. |
| molecularNote | The "Promoterless-lacZ" transgene (pEMS1313) was designed with no Pleiades minipromoter upstream of a frt-flanked _-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. Without a promoter directing expression of beta-galactosidase (lacZ), no lacZ expression pattern is seen or anticipated. |
