| First Author | Wu K | Year | 2018 |
| Journal | J Cell Sci | Volume | 131 |
| Issue | 22 | PubMed ID | 30333138 |
| Mgi Jnum | J:266974 | Mgi Id | MGI:6257372 |
| Doi | 10.1242/jcs.221036 | Citation | Wu K, et al. (2018) GCN5L1 interacts with alphaTAT1 and RanBP2 to regulate hepatic alpha-tubulin acetylation and lysosome trafficking. J Cell Sci 131(22):jcs221036 |
| abstractText | Although GCN5L1 (also known as BLOC1S1) facilitates mitochondrial protein acetylation and controls endosomal-lysosomal trafficking, the mechanisms underpinning these disparate effects are unclear. As microtubule acetylation modulates endosome-lysosome trafficking, we reasoned that exploring the role of GCN5L1 in this biology may enhance our understanding of GCN5L1-mediated protein acetylation. We show that alpha-tubulin acetylation is reduced in GCN5L1-knockout hepatocytes and restored by GCN5L1 reconstitution. Furthermore, GCN5L1 binds to the alpha-tubulin acetyltransferase alphaTAT1, and GCN5L1-mediated alpha-tubulin acetylation is dependent on alphaTAT1. Given that cytosolic GCN5L1 has been identified as a component of numerous multiprotein complexes, we explored whether novel interacting partners contribute to this regulation. We identify RanBP2 as a novel interacting partner of GCN5L1 and alphaTAT1. Genetic silencing of RanBP2 phenocopies GCN5L1 depletion by reducing alpha-tubulin acetylation, and we find that RanBP2 possesses a tubulin-binding domain, which recruits GCN5L1 to alpha-tubulin. Finally, we find that genetic depletion of GCN5L1 promotes perinuclear lysosome accumulation and histone deacetylase inhibition partially restores lysosomal positioning. We conclude that the interactions of GCN5L1, RanBP2 and alphaTAT1 function in concert to control alpha-tubulin acetylation and may contribute towards the regulation of cellular lysosome positioning. This article has an associated First Person interview with the first author of the paper. |
