First Author | Belin D | Year | 1985 |
Journal | Eur J Biochem | Volume | 148 |
Issue | 2 | Pages | 225-32 |
PubMed ID | 2985383 | Mgi Jnum | J:7809 |
Mgi Id | MGI:56278 | Doi | 10.1111/j.1432-1033.1985.tb08829.x |
Citation | Belin D, et al. (1985) Cloning, nucleotide sequencing and expression of cDNAs encoding mouse urokinase-type plasminogen activator. Eur J Biochem 148(2):225-32 |
abstractText | Controlled extracellular proteolysis is catalyzed in part by the secretion of plasminogen activators. As a step in the study of the expression of these enzymes in mouse tissues, we have isolated five cDNAs encoding the mouse urokinase-type plasminogen activator from a cDNA library prepared with size-selected mRNA from MSV-transformed 3T3 cells. The longest cDNA insert contains the entire coding region of mouse urokinase, 58 base pairs of the 5' non-coding region, and the entire 3' non-coding region, which is 942 base pairs long. The deduced protein sequence, which starts with a signal peptide of 20 amino acids, shows extensive homology to that of human and porcine urokinase. However, in contrast to these enzymes, mouse urokinase contains no N-glycosylation site. Bacteria harbouring one of the recombinant plasmids synthesize and secrete into their periplasm a protease indistinguishable from mouse urokinase. |