| First Author | Loya F | Year | 1994 |
| Journal | Blood | Volume | 84 |
| Issue | 6 | Pages | 1831-6 |
| PubMed ID | 8080988 | Mgi Jnum | J:20716 |
| Mgi Id | MGI:68788 | Doi | 10.1182/blood.v84.6.1831.1831 |
| Citation | Loya F, et al. (1994) Transgenic mice carrying the erythropoietin gene promoter linked to lacZ express the reporter in proximal convoluted tubule cells after hypoxia. Blood 84(6):1831-6 |
| abstractText | Results on the localization of erythropoietin (Epo) synthesis in renal cells have been contradictory, implicating either interstitial or tubular cells. We fused the lacZ gene to a 7-kb DNA fragment of the mouse Epo gene encompassing a portion of the first intron, the first exon, and a 6-kb sequence of the 5'-flanking region. Transgenic mice carrying this construct show a low level of specific expression of the lacZ gene in proximal convoluted tubule (PCT) cells. Without hypoxia, no significant expression was detected in the liver. Hypoxia induced a large degree of lacZ expression, mainly in kidney PCT cells and to a lesser degree in liver. However, anemia induced lacZ expression in both kidney and liver. These findings indicate that, under these conditions, Epo is expressed in tubular cells, specifically PCT cells. |
