| First Author | Tagoh H | Year | 1996 |
| Journal | Biochem Biophys Res Commun | Volume | 221 |
| Issue | 3 | Pages | 744-9 |
| PubMed ID | 8630032 | Mgi Jnum | J:33663 |
| Mgi Id | MGI:81141 | Doi | 10.1006/bbrc.1996.0667 |
| Citation | Tagoh H, et al. (1996) Molecular cloning and characterization of a novel stromal cell-derived cDNA encoding a protein that facilitates gene activation of recombination activating gene (RAG)-1 in human lymphoid progenitors. Biochem Biophys Res Commun 221(3):744-9 |
| abstractText | The activation and expression of recombination activation genes (RAGs) in lymphoid progenitors are regulated by signals from surface molecules of stromal cells and/or cytokines. Using a mRNA differential display method, we isolated a novel stromal cell-derived cDNA clone, C2.3, whose transcripts were intensively expressed in RAG-1-inducible stromal cell line, but rarely expressed in RAG-1-non inducible mutant cell line (PA6). The cDNA sequence had no homology to the known genes. The sequence revealed an open reading frame that encodes a 221 amino acid protein with 4 potential transmembrane domains, suggesting a possible role of C2.3 product as a membrane receptor. Introduction of C2.3 cDNA into PA6 mutant line restored the ability to activate RAG-1 gene in lymphoid progenitors, indicating that a C2.3 product may be involved in the induction of RAG-1 gene activation. |
