| First Author | Mori T | Year | 2004 |
| Journal | FEBS Lett | Volume | 557 |
| Issue | 1-3 | Pages | 209-14 |
| PubMed ID | 14741369 | Mgi Jnum | J:87559 |
| Mgi Id | MGI:3027174 | Doi | 10.1016/s0014-5793(03)01495-9 |
| Citation | Mori T, et al. (2004) NIRF is a ubiquitin ligase that is capable of ubiquitinating PCNP, a PEST-containing nuclear protein. FEBS Lett 557(1-3):209-14 |
| abstractText | We previously reported the association of a novel Np95/ICBP90-like RING finger protein (NIRF) with a novel PEST-containing nuclear protein (PCNP). NIRF is a nuclear protein with a ubiquitin-like domain, a PHD finger, a YDG/SRA domain, Rb-binding motifs and a RING finger. In this study, we showed that NIRF has auto-ubiquitination activity, the hallmark of a ubiquitin ligase. PCNP was readily ubiquitinated in 293 and COS-7 cells, and NIRF ubiquitinated PCNP in vitro as well as in vivo. Considering that NIRF is implicated in cell cycle regulation, these findings suggest that NIRF and PCNP are a ubiquitin ligase and its substrate, respectively, and may constitute a novel signaling pathway with some relation to cell proliferation. |
