| First Author | Majewski M | Year | 2007 |
| Journal | Int Immunol | Volume | 19 |
| Issue | 6 | Pages | 719-32 |
| PubMed ID | 17446207 | Mgi Jnum | J:122307 |
| Mgi Id | MGI:3713979 | Doi | 10.1093/intimm/dxm034 |
| Citation | Majewski M, et al. (2007) Protein kinase C delta stimulates antigen presentation by Class II MHC in murine dendritic cells. Int Immunol 19(6):719-32 |
| abstractText | Maturation of dendritic cells (DCs) regulates protein sorting in endosomal compartments to promote the surface expression of molecules involved in T cell activation. MHC Class II complexes are mobilized to the surface via intracellular effector molecules that remain largely unknown. We here show that protein kinase C (PKC) stimulates Class II antigen surface expression, using knock-in mice that express a Class II-green fluorescent protein fusion protein as a read out. Selective inhibition of PKCdelta counteracts the ability of DCs to stimulate Class II MHC-restricted antigen-specific T cells. Activation of PKC does not affect antigen uptake, peptide loading and surface display of Class I MHC and transferrin receptor in DCs. We show that activation-induced Class II MHC surface expression is dependent on activation of PKCdelta and conclude that this event is pivotal for optimal CD4 T cell activation. |
