| First Author | Lok S | Year | 1985 |
| Journal | Mol Cell Biol | Volume | 5 |
| Issue | 9 | Pages | 2221-30 |
| PubMed ID | 3837188 | Mgi Jnum | J:8369 |
| Mgi Id | MGI:56836 | Doi | 10.1128/mcb.5.9.2221 |
| Citation | Lok S, et al. (1985) Lens-specific promoter activity of a mouse gamma-crystallin gene. Mol Cell Biol 5(9):2221-30 |
| abstractText | Crystallins are the major water-soluble proteins in vertebrate eye lenses. These lens-specific proteins are encoded by several gene families, and their expression is differentially regulated during lens cell differentiation. Here we show that a cloned mouse gamma-crystallin promoter is active in lens explants derived from 14-day-old chicken embryos but inactive in a variety of cells of non-lens origin. We also show that sequences required for proper utilization of this promoter are contained between nucleotide positions -392 and +47 relative to the transcription initiation site; deletion of sequences from positions -392 to -171 completely abolishes promoter activity. Since chickens do not have gamma-crystallin genes, the expression of a mouse gamma-crystallin promoter in chicken lens cells suggests that different classes of crystallin genes may be regulated by common lens tissue-specific mechanism(s) independent of species. |
