| Primary Identifier | MGI:5825017 | Allele Type | Targeted |
| Attribute String | Conditional ready, Null/knockout, Reporter | Gene | Slc6a8 |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129P2/OlaHsd)F1 |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cells used for allele generation: clone A8, gift fromA. Wutz, Wellcome Trust Centre for Stem Cell Research, Stem Cell Institute, University of Cambridge (J:232606). |
| molecularNote | This KO first allele (reporter-tagged insertion with conditional potential) was generated using EUCOMM vector (PCS00081_A09). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons. The critical exons 5 to 7 are thus flanked by loxP sites. For vector details, see Slc6a8tm1a(KOMP)Wtsi. |
