| Type | MGI:General | Description | after intracytoplasmic sperm injection (ICSI), only ~15% of wild-type oocytes injected with mutant sperm develop to 2-cell embryos, indicating fertilization failure after ICSI after artificial oocyte activation (AOA) treatment, the 2-cell embryo rate in the ICSI group injected with mutant sperm is increased from ~15% to ~40% failure to initiate Ca2+ oscillations in ICSI embryos with mutant sperm contributes to fertilization failure after ICSI even after AOA treatment, only 2 out of total 73 embryos injected with mutant sperm reach the blastocyst stage, suggesting that additional sperm defects affect the in vitro developmental potential of zygotes, and these are not rescued by ICSI-AOA |
