First Author | Seki N | Year | 1999 |
Journal | J Hum Genet | Volume | 44 |
Issue | 3 | Pages | 185-9 |
PubMed ID | 10319584 | Mgi Jnum | J:55223 |
Mgi Id | MGI:1337516 | Doi | 10.1007/s100380050139 |
Citation | Seki N, et al. (1999) Cloning, tissue expression, and chromosomal assignment of human MRJ gene for a member of the DNAJ protein family. J Hum Genet 44(3):185-9 |
abstractText | The DnaJ protein family consists of proteins with a highly conserved amino acid stretch called the J-domain. A cDNA clone encoding a new protein with a J-domain was isolated from a human fetal brain cDNA library. This new member of the DnaJ family of 241 amino acid residues showed 94% identity with mouse Mrj (accession number, AF035962) and 71% identity with mouse Msj-1 (accession number, U95607) along its entire sequence. Reverse transcription-coupled polymerase chain reaction (RT-PCR) analysis showed the messenger RNA was ubiquitously expressed in various human tissues. The chromosomal location of the gene was determined by PCR-based analyses with both a human/rodent monochromosomal hybrid cell panel and a radiation hybrid panel to map on chromosome 11q25 region. |