| Primary Identifier | MGI:6368177 | Allele Type | Targeted |
| Attribute String | Inducible, Knockdown, Reporter | Gene | Col1a1 |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Induced With | doxycycline/tetracycline | Is Recombinase | false |
| Is Wild Type | false |
| description | The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae. This allele was generated with Col1a1tm13(neo/hygro*)Jae. |
| molecularNote | RMCE (recombinase-mediated cassette exchange) was utilized to insert tetO-GFP/RNAi:Renilla luciferase (rluc) into the Col1a1 locus. The vector contains a tetracycline responsive promoter (TRE;tetO) controlling expression of a GFP reporter and short-hairpin RNA sequence targeting luciferase embedded in the MIR30 structure. The vector was then co-electroporated with a FLPe recombinase-expressing vector into the RMCE-ready KH2 ES cells. |
