| First Author | Jung U | Year | 1998 |
| Journal | Am J Physiol | Volume | 274 |
| Issue | 5 Pt 2 | Pages | H1785-91 |
| PubMed ID | 9612391 | Mgi Jnum | J:47723 |
| Mgi Id | MGI:1205963 | Doi | 10.1152/ajpheart.1998.274.5.H1785 |
| Citation | Jung U, et al. (1998) Gene-targeted mice reveal importance of L-selectin-dependent rolling for neutrophil adhesion. Am J Physiol 274(5 Pt 2):H1785-91 |
| abstractText | It has not been determined whether L-selectin-mediated rolling can promote leukocyte adhesion in vivo independent of P- and E-selectin. We used intravital microscopy of E- and P-selectin double-mutant mice (E-/P-) stimulated with tumor necrosis factor-alpha for 6-8 h to investigate the importance of L-selectin-dependent rolling in cremaster muscle venules. Rolling leukocyte flux in E-/P- mice was 9 +/- 2 cells/min compared with 77 +/- 17 cells/min in wild-type (WT) mice. Pre-treatment with the L-selectin monoclonal antibody MEL-14 significantly reduced rolling in both E-/P- (by 89%) and WT mice (by 79%). L-selectin-dependent rolling in E-/P- mice resulted in leukocyte adhesion comparable to that seen in WT mice. MEL-14 pretreatment of E-/P- mice reduced leukocyte adhesion by 50%. The majority (approximately 80%) of intravascular leukocytes in both WT and E-/P- mice were neutrophils. We conclude that L-selectin can mediate rolling that results in sufficient leukocyte recruitment to account for the robust inflammatory response seen in E-/P- mice at later times. |
