| Primary Identifier | MGI:3528558 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Csmd1 |
| Transmission | Germline | Strain of Origin | 129S5/SvEvBrd |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | Lexicon |
| molecularNote | The coding portion of the first exon along with some first intron sequence was deleted and a selection cassette was inserted in its place after the homologous recombination event. This selection cassette contains a Beta-Galactosidase marker (LacZ) and a Neomycin resistance (Neo) fragment to allow selection in ES cells. qPCR confirmed reduced expression of transcripts containing exon 1 and 2 in the brain. Western blot analysis confirmed the absence of full-length protein expression in the cortex. |
