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Allele : Sftpc<tm2.1(NtTA)Thbr> surfactant associated protein C; targeted mutation 2.1, Thomas Braun

Primary Identifier  MGI:7263085 Allele Type  Targeted
Attribute String  Epitope tag, Reporter, Transactivator Gene  Sftpc
Transmission  Germline Strain of Origin  (C57BL/6 x 129S4/SvJae)F1
Is Recombinase  false Is Wild Type  false
molecularNote  Mice were generated by inserting a 2A sequence, YFP, 2A sequence, and the N-terminal fragment of tTA (aa 1-205) fused to the N terminal 102 amino acids of the DnaE split intein sequence from Nostoc punctiforme (Npu), FLAG tag, and Frt-flanked neomycin resistance cassette immediately in front of the stop codon of Sftpc coding sequence by homologous recombination. The Frt-flanked neomycin resistance cassette was removed by crossing with hACTB-Flpe deleter mice.
  • mutations:
  • Insertion
  • synonyms:
  • SPC<-2A-YFP-2A-tTA-N>,
  • SPC<-2A-YFP-2A-tTA-N>
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1 Feature

Trail: Allele

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

0 Carried By

0 Driven By

3 Publication categories

Trail: Allele