Primary Identifier | MGI:7263085 | Allele Type | Targeted |
Attribute String | Epitope tag, Reporter, Transactivator | Gene | Sftpc |
Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Mice were generated by inserting a 2A sequence, YFP, 2A sequence, and the N-terminal fragment of tTA (aa 1-205) fused to the N terminal 102 amino acids of the DnaE split intein sequence from Nostoc punctiforme (Npu), FLAG tag, and Frt-flanked neomycin resistance cassette immediately in front of the stop codon of Sftpc coding sequence by homologous recombination. The Frt-flanked neomycin resistance cassette was removed by crossing with hACTB-Flpe deleter mice. |