| Primary Identifier | MGI:6188634 | Allele Type | Chemically induced (ENU) |
| Attribute String | Hypomorph | Gene | Tpi1 |
| Inheritance Mode | Recessive | Strain of Origin | SJL |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | ENU mutagenesis induced a T to C mutation at position c170 in the first exon, resulting in a phenylalanine to serine mutation at amino acid 57. RT-PCR shows no difference in mRNA expression levels compared to wild-type, however, Western blot analysis indicates a significant reduction of protein in the bone marrow of homozygotes. TPI1 enzyme activity is 10%, 12%, 13% and 16% of wild-type levels in red blood cells, in the brain, in bone marrow-derived macrophages, and in unsorted bone marrow, respectively. |
