First Author | Stafford DA | Year | 2017 |
Journal | Proc Natl Acad Sci U S A | Volume | 114 |
Issue | 1 | Pages | 101-106 |
PubMed ID | 27986952 | Mgi Jnum | J:239387 |
Mgi Id | MGI:5828678 | Doi | 10.1073/pnas.1612069113 |
Citation | Stafford DA, et al. (2017) Deletion of the sclerotome-enriched lncRNA PEAT augments ribosomal protein expression. Proc Natl Acad Sci U S A 114(1):101-106 |
abstractText | To define a complete catalog of the genes that are activated during mouse sclerotome formation, we sequenced RNA from embryonic mouse tissue directed to form sclerotome in culture. In addition to well-known early markers of sclerotome, such as Pax1, Pax9, and the Bapx2/Nkx3-2 homolog Nkx3-1, the long-noncoding RNA PEAT (Pax1 enhancer antisense transcript) was induced in sclerotome-directed samples. Strikingly, PEAT is located just upstream of the Pax1 gene. Using CRISPR/Cas9, we generated a mouse line bearing a complete deletion of the PEAT-transcribed unit. RNA-seq on PEAT mutant embryos showed that loss of PEAT modestly increases bone morphogenetic protein target gene expression and also elevates the expression of a large subset of ribosomal protein mRNAs. |