| First Author | Calvo CF | Year | 1991 |
| Journal | J Immunol | Volume | 146 |
| Issue | 4 | Pages | 1353-60 |
| PubMed ID | 1991972 | Mgi Jnum | J:10972 |
| Mgi Id | MGI:59414 | Doi | 10.4049/jimmunol.146.4.1353 |
| Citation | Calvo CF, et al. (1991) DNA sequences 3' of the Ig H chain cluster rearrange in mouse B cell lines. J Immunol 146(4):1353-60 |
| abstractText | A mouse myeloma cell line MPC11 (IgG2b, kappa) and variants derived from it have been used to study DNA rearrangements that occur at the Ig H chain locus. One variant, F5.5, has acquired both VH gene and C epsilon gene rearrangements. Through genomic Southern blot analysis initially directed to mapping the C epsilon gene rearrangement, we observed that the VH region rearrangement was linked, through an inversion event, to sequences that originate 3' of the CH cluster, i.e., 3' of the C alpha gene. Subsequent studies have shown that DNA rearrangements within the region 3' of the C alpha gene are detected in several other mouse myeloma and hybridoma cell lines and are not associated with the expression of specific isotypes. |
